Immunochromatographic assay for detection of tropomyosin of fish from food.

Limit of detection:

0.5 ppm

Internal validation following International guidelines:
Appendix M: Validation Procedures for Quantitative. Food Allergen ELISA Methods: Community Guidance and Best Practices. AOAC 2012 (http://www.eoma.aoac.org/app_m.pdf).
Appendix F: Guidelines for Standard Method Performance Requirements. Official Methods of Analysis (2016), AOAC INTERNATIONAL, Rockville, MD, USA (http://www.eoma.aoac.org/app_f.pdf)
Standard Method Performance Requirements (SMPRs®) for Detection and Quantitation of Selected Food Allergens. AOAC SMPR 2016.002. AOAC INTERNATIONAL, Rockville, MD, USA (https://www.aoac.org/aoac_prod_imis/AOAC_Docs/SMPRs/SMPR%202016_002.pdf).
Standard Method Performance Requierements (SMPRs®) for Quantitation of Wheat, Rye and Barley Gluten in Oats. AOAC SMPRs® 2017.021 (http://members.aoac.org/aoac_prod_imis/AOAC_Docs/ISPAM/SMPR_2017.021.pdf)