MULLER-KAUFFMANN TETRATHIONATE-NOVOBIOCIN BROTH (MKTTN) (without iodine)

P/N: BK208HA

Application:	MKTTn Broth is used as one of two selective enrichment medium, along with RVS broth, for salmonellae in milk and dairy products and in other food products following the horizontal method described in the ISO 6579 standard. MKTTn broth is also used as a second selective enrichment broth for the detection of Salmonella in waters.
Microorganisms:	Salmonella
Formula:	For 1025 mL of media: - Tryptone 8,6 g - Meat extract 4,3 g - Bile salts 4,78 g - Sodium chloride 2,6 g - Calcium carbonate 38,7 g - Sodium thiosulfate, anhydrous 30,45 g - Brillant green 9,6 mg - Iodine 4,0 g - Potassium Iodide 5,0 g - Novobiocin 40 mg pH of the ready-to-use media at 25 °C: 7.0-8.0
Quantity for preparation of 1 L:	89,5 g
Sterilization:	Do not autoclave; boil for 2 min.
Addition:	This broth does not contain Iodine-iodide, add separately.
Inoculation:	1 mL in 10 mL
Incubation:	24 ± 3 hours: - at 36 ± 2 °C for detection of Salmonellae in water; - at 34-38°C for the detection of Salmonellae in food; - at 41.5 ± 1 °C for the detection of Salmonellae in an animal health context.
Packing:	500 g